Procaidin B2 Hauv Apples: A 275ug / G Powerhouse rau Lub Plawv Noj Qab Haus Huv?

Niaj hnub no txoj kev nrhiav kev noj qab haus huv thiab cov khoom lag luam, cov nroj tsuag extract kev lag luam yog kev vam meej. Raws li cov txiv hmab txiv ntoo txhua hnub, txiv apples muaj cov khoom noj khoom haus muaj txiaj ntsig deb dhau ntawm peb txoj kev xav, tshwj xeeb tshaj yog cov khoom siv nquagProcyanidin B2muaj nyob hauv lawv. Kua polyphenols feem ntau yog muaj li ntawm phenolic acid Cheebtsam, suav nrog inehocyanins, anthocyanins, flavonoids, thiab chlorogenic acid._[1]Ntawm lawv, Anthocyanins tus account rau 71% rau 90% ntawm polyphenol cov ntsiab lus hauv txiv apples, uas yog cov khoom xyaw nquag tshaj plaws._[2]Proantocyanins muaj dimers tsim los ntawm catechins lossis epicatecins, ntxiv rau cov ntxuag, tetramers, thiab txawm tias decamers. Raws li cov℃ntawm polymerization, ob mus rau plaub oligomers raug xa mus ua Odigomeric Proantamersan, thiab ntau dua yog raug xa mus ua polymeric proantocyanidins.

1.1 Nrig muab faib tsis ncaj
Kev faib tawm ntawmProcyanidin B2Hauv cov txiv apples qhia qhov tsis xwm yeem tseem ceeb. Kev tshawb fawb tau pom tias muaj qee qhov sib txawv hauv cov ntsiab lus ntawm Procyanidin B2 hauv ntau qhov chaw ntawm txiv apples. Cov txiaj ntsig kev txiav txim siab ntawm ntau hom Apple ntau yam uas siv cov ntsiab lus HPLC pom tias cov ntsiab lus ntawm Proxyanidin B2 hauv Apple Ranged los ntawm 275.24 txog 548.42 μ g / g g / g g / g. Hauv kev sib piv, cov ntsiab lus nyob rau hauv cov txiv hmab txiv ntoo ntxaij teb los ntawm 90.19 rau 247.06 μ g / g. Qhov sib txawv no yog feem ntau vim yog cov haujlwm sib txawv ntawm lub cev thiab cov kev ua si metabolic ntawm ntau qhov chaw Apple._[4] 

1.2 Qhov sib txawv ntawm ntau yam
Muaj ntau qhov sib txawv hauv cov ntsiab lus ntawmProcyanidin B2Ntawm cov kua sib txawv ntau yam. Kev kawm ntawm tsib ntau yam ntawm cov khoom siv tau pom tias cov ntsiab lus ntawm Proxyanidin B2, Gala (548.23 μ g), thiab kua ntsuab (275.24 G g / g)._[4]Cov kev faib khoom tawg ntawm Polyphenol cov ntsiab lus hauv Suav teb cov peev txheej hauv Suav teb qhia tau tias lawv cov ntsiab lus polyphenol txawv thiab lawv txoj kev sib txawv yog nplua nuj._[5]Nyob rau hauv rau rau me me kua ntau yam nyob rau hauv Tebchaws Northeast Suav, cov kws tshawb nrhiav tau ntes 12 Hom Cheeb Tsam Flavonid Cheebtsam, suav nrog Proyanidin B1, B2, thiab B4. Cov ntsiab lus ntawm cov ntau yam no tau tshaj tawm los ntawm 10.20 txog 73.77 mg / kg, nrog flavanols ua si lub luag haujlwm ntawm flavonoid._[6]

1.3 kev cuam tshuam ntawm cov khoom hauv cheeb tsam
TusProcyanidin B2Cov ntsiab lus hauv cov txiv av kuj yog nyob ntawm thaj av loj hlob. Kev tshawb fawb tau pom tias cov ntsiab lus ntawm flavonols thiab anthocyanins hauv North America yog tsawg, anthology acid, thiab dihydrochalcone yog ib nrab. Cov ntsiab lus ntawm tsib hom polyphenolic tshuaj nyob rau hauv cov khoom noj qus Apple los ntawm qib Lavxias, thiab Tuam Tshoj cov tsiaj qus cov khoom siv muaj ntau haiv neeg nplua nuj.

2.1 high {- Kev ua si kua chromatogy txoj kev
Tam sim no, siab - Kev ua kua chromatography (HPLC) yog ib txoj kev siv rau kev txiav txim siab ntawm Procyanidin B2 hauv txiv apples. Kev paub raug mob muaj xws li cov neeg mob muaj xws li: ib qho xwm txheej ntawm C18 (250 hli × 4.6mm, 5 μ m) raws li cov chromatographic kem; Txawb theem a yog ib qho yog 0.5% phosphoric acid aclutrol, thiab theem b yog dej acetonitrile (50:50, v / v); Txaus tus nqi ntawm 1.0ml / min; Sab kub kub 30 degree; Qhov kev pom zoo yog 280nM6. Cov qauv no ua rau pom zoo heev linearity (r²=0.9998}), nrog ib tus nqi rov qab ntawm 97.72% thiab raug zoo._[4]

2.2 ultra -} kev ua kom zoo ua kua chromatography ua ke nrog huab hwm coj hluav taws xob
Ultra -} Kev Ua Haujlwm Siab Ua Kua chromoography txuas nrog huab hwm coj zoo nkauj (UPLC} MS) tau los kuj tau thov rau hauv kev kawm ntawm Apple Polyphenols. Ib qho kev tshawb fawb siv hom no los txiav txim cov polyphenol muaj pes tsawg leeg thiab cov khoom siv tsiaj qus thiab Asmeskas thiab 22 polyphenolies tshuaj._[5]

3.1 antioxidant muaj zog
Procyanidin B2muaj cov tshuaj tiv thaiv kev tiv thaiv kev tiv thaiv zoo heev. Kev tshawb fawb tau pom tias nyob rau hauv tsis -} f} Cov kua txiv, anthocyanins yog lub peev xwm tseem ceeb rau hauv vitro antioxidant muaj peev xwm.Procyanidin B2, Epicatechin, thiab Epicatechin Gallate, muab tso tawm dpph muaj zog dpph radical scavenging muaj peev xwm ntau dua ntawm tag nrho cov ntsiab lus phenolic._[7]

3.2 anti {-} mob thiab anti -} Cov khoom siv txhua yam
ProCyanidin B2 tseem ceeb toom cov khoom lag luam tseem ceeb anti - o}} {{{{{}} kev ua txhua yam. Cov kws tshawb fawb tshawb fawb ntawm South Suav teb Botanical Vaj Muaj cov lus qhia txog kev ua yeeb yam zoo li cov khoom xyaw rau lub tebchaws, thiab tau txais cov tshuaj muaj zog patent. Qhov no muaj cov khoom ruaj khov thiab haum rau siv nyob rau hauv cov khoom lag luam tawv nqaij zoo li nws tsis} {{{{overgenating, anti anti - -}

3.3 Kev Tiv Thaiv Ntshav Qab Zib thiab Lub Siab Ua Haujlwm
ProanticoSanidins (suav nrogProcyanidin B2) Cov txiv av tau ua rau muaj kev tiv thaiv cov kabmob ntshav qab zib thiab daim siab tsis sib xws. Ua ib qho tshuaj tiv thaiv ntuj, anthocyanins muaj ntau cov roj ntsha ua ke xws li anti {{otion} Cov kev sim tsiaj pom tias cov mice kev sim pub nrog procyanidins pom tias muaj kev txhim kho tseem ceeb ntawm cov ntshav qab zib thiab lub siab ua tsis tau.

4.1 Cov Khoom Noj thiab Khoom Noj Qab Haus Huv 4.1
Procyanidin B2muaj kev cia siab thov cov kev cia siab hauv cov chaw ua noj ua haus thiab cov khoom noj qab haus huv. Nrog rau qhov kev thov nce zuj zus rau cov khoom siv noj qab haus huv, Apple extract Riches hauv Procyanidin B2 tuaj yeem ntxiv ua cov tshuaj tiv thaiv ntuj rau ntau yam khoom noj thiab cov khoom noj qab haus huv. Siv ob qho txiv apples ib hnub twg tuaj yeem muaj cov txiaj ntsig zoo ntawm lub hauv paus kev lag luam lub plawv, muab kev tshawb fawb rau kev txhim kho cov khoom noj muaj zog {}}}}

4.2 Tshuaj pleev ib ce thiab cov khoom siv saib xyuas tus kheej
Hauv cov teb ntawm cov tshuaj pleev ib ce thiab cov khoom siv saib xyuas tus kheej,Procyanidin B2kuj qhia tau muaj peev xwm loj. Lub compound natural whitening thiab anti {- wrinkle muaj pes tsawg daim ntawv tso cai. Qhov kev sib xyaw muaj ntau yam, xws li tsis {{}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} {}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}

4.3 Cov Kev Siv Kho Mob
Ib qho ntxiv, nws muaj peev xwm tiv thaiv kev mob ntshav qab zib thiab lub siab yuav muaj nqis kev tshawb fawb ntxiv thiab kev txhim kho.

Txawm yogProcyanidin B2Hauv cov txiv apples muaj ntau cov txiaj ntsig kev noj qab haus huv thiab thov kev cia siab, nws txoj kev txhim kho thiab kev siv tseem fim qee cov kev cov nyom:

• Extraction txheej txheem optimization: nws yog ib qho tsim nyog los txhim kho ntau cov txheej txheem los txhim kho cov txheej txheem rho tawm thiab purity ntawm procanidin b2.
• Qhov teeb meem ruaj ntseg: ProCyanidin B2 yog ib txwm muaj kev ua tsis muaj kev cuam tshuam thaum lub sijhawm ua thiab kev cia khoom, thiab cov qauv kev tiv thaiv thiab siv tau zoo.
• Bioaccumulation: Yuav ua li cas txhim kho cov xyoob bioavailability ntawm Procyanidin B2 yog qhov teeb meem tseem ceeb uas xav tau hais.
• Cov Qauv Qauv thiab Kev Tswj Xyuas Zoo: Nws yog qhov tsim nyog los tsim cov qauv kev ntsuas zoo qauv kom ntseeg tau tias qhov ruaj khov thiab kev ntseeg tau ntawm cov khoom.

Kev tshawb nrhiav yav tom ntej yuav tsum tsom mus rau txoj kev nkag siab tob txog cov txheej txheem ntawm kev ua ntawmProcyanidin B2, tsim kev loj - tus nqi -}}}}}}}} Cov khoom siv tau ntxiv hauv ntau daim teb.

Procyanidin B2, raws li ib qho tseem ceeb bioactive nyob rau hauv cov txiv av, ua pov thawj tsis sib luag thiab muaj feem ntau los ntawm ntau yam xws li ntau yam, thiab thaj chaw. Nws cov txiaj ntsig zoo li no, xws li kev tiv thaiv kev noj qab haus huv, tiv thaiv anti {{{{1} {{1} {{1} {{1} {{1} {{1} {{1} {{ Nrog kev nce qib nruam ntawm cov nroj tsuag extract thev naus laus zis thiab kev txhim kho ntawm cov neeg siv khoom, kev txhim kho ntawm cov khoom siv presanidin B2 hauv txiv apples yuav ua rau cov hauv kev tshiab. Yog xav paub cov ntsiab lus ntxiv, thov hu rau SERRYA. (Email:cwj@appchem.cn)

Siv:

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[2] Vrhovsek u, rigo a, tonon d, li al. Txawm tias muaj nuj nqis ntawm polyphenols hauv cov hom sib txawv [j]. Aglic zaub mov zaub mov chem, 2004, 52 (21): 6532-6538.
[3] Yang Jingyu, Lis Yan, Wang Fang, li al. Hepatoprotective cuam tshuam ntawm cov kua polyphenols ntawm CCL4-ua rau lub siab mob rau nas [j]. Agic zaub mov noj tshuaj chem, 2010, 58 (10): 6525-6531.

[4] Wang Jiao, Zaj Xinbo, thov nkauj nyab nraug. HPLC kev txiav txim siab ntawm Proanthocyanidin B2 hauv ntau hom ntawm cov txiv apples [j]. Khoom Noj Khoom Haus Kawm Txuj Ci, 2012, 33 (24): 295.

[5] Wang Dajiang, Wang Kun, Li Jing. Kev kawm ntawm Polyphenol cov ntsiab lus hauv cov txiv hmab txiv ntoo ntawm 20 cov khoom siv tsiaj qus [J]. Suav txiv hmab txiv ntoo ntoo, 2017, (04): 74-78. DOI: 10.16626 / J.cnki.issn1000-8047.2017.04.025.
[6] Liu Chang, Zhao Jirong, Wang Kun. Kev tsom xam ntawm flavonoid Cheebtsam thiab cov ntsiab lus ntawm cov txiv hmab txiv ntoo sib txawv hauv Suav teb hauv Northeast Suav [J]. Hav zoov los ntawm - cov khoom thiab tshwj xeeb hauv Suav teb, 2020, (05): 25-28. DOI: 10.13268 / J.CKKI.FBKEIC.2020.007.

[7] Wang Yangi, Gu Yurong, Wang Yongtao. Kev txheeb xyuas ntawm phenolic muaj pes tsawg leeg tshuaj thiab antioxidant cov haujlwm ntawm NFC Kua kua kua kua kua los ntawm txawv cultivars [j]. Phau ntawv Journal ntawm Suav lub koom haum ntawm zaub mov science thiab thev naus laus zis, 2020, 20 (05): 74-83. DOI: 10.16429 / j.1009-7848.2020.05.010.010.