Tyrosinase, raws li tus nqi tseem ceeb - txwv enzyme nyob rau hauv txoj kev sib txuas ntawm melanin, yeej ib txwm yog lub hom phiaj tseem ceeb hauv kev tsim cov khoom lag luam. Nyob rau hauv xyoo tas los no,Phloretin, lub ntuj polyphenol muab tau los ntawm cov nroj tsuag xws li txiv apples thiab pears, tau tshwm sim nyob rau hauv daim ntawv kho mob ntawm daim tawv nqaij vim nws cov antioxidant zoo heev thiab kev nkag mus rau - txhawb lub peev xwm.
1, Kev tshawb fawb pov thawj thiab kev tsis sib haum xeeb: Tshawb xyuas cov txheej txheem uasPhloretininhibits tyrosinase
Qhov kev pom zoo ntawm kev kawm txog seb Phloretin tuaj yeem cuam tshuam tyrosinase tsis zoo ib yam, uas qhia meej txog qhov nyuaj thiab nyuaj ntawm kev tshawb fawb tshawb fawb.
- Core scientific discovery: ib tug reversible mixed inhibitor
Ib txoj kev tshawb fawb tseem ceeb luam tawm xyoo 2020 muab cov pov thawj ruaj khov rau kev ua haujlwm inhibitory ntawmPhloretin. Daim ntawv "Phloretin ua ob qho tib si substrate thiab inhibitor ntawm tyrosinase: Inhibition kev ua thiab mechanism," luam tawm los ntawm Jianmin Chen li al. Hauv Food Chemistry, qhia tias Phloretin nthuav tawm cov nyhuv inhibitory ntawm nceb tyrosinase. Txoj kev tshawb fawb tau txiav txim siab los ntawm kev tshuaj ntsuam kinetic tias Phloretin yog ib qho kev sib xyaw ua ke ntawm cov tshuaj tiv thaiv nrog IC50 tus nqi ntawm 169.36 μ mol / L.[1,5]
'Mixed inhibition' txhais tau tiasPhloretintuaj yeem khi rau ob qho tib si dawb tyrosinase thiab enzyme substrate complexes uas twb tau khi rau cov substrates (xws li L-tyrosine), yog li thaiv txoj hauv kev ntawm melanin ntau lawm ntawm ntau theem. Dab tsi yog qhov nthuav ntxiv yog tias txoj kev tshawb fawb kuj pom tias Phloretin nws tus kheej tuaj yeem ua lub substrate rau tyrosinase. Qhov tshwj xeeb no "substrate inhibitor" dual tus kheej ua rau nws tsim kev ruaj khov nrog cov enzyme, raws li tau lees paub los ntawm kev sim fluorescence quenching.[4] Qhov no qhia tau hais tias kev sib cuam tshuam ntawm Phloretin thiab tyrosinase yog qhov tseeb thiab ruaj khov, tsis yog qhov tshwm sim random sim.
- Kev txheeb xyuas cov ntaub ntawv sib txawv thiab kev tsis sib haum xeeb
Txawm hais tias cov kev tshawb fawb saum toj no muab pov thawj tseeb, peb kuj yuav tsum lees paub qee cov ntaub ntawv tsis sib haum xeeb hauv kev lag luam. Qee cov ntaub ntawv tshawb fawb qhia tiasPhloretintsis muaj kev cuam tshuam inhibitory tiv thaiv tyrosinase nyob rau hauv cov kev sim tshwj xeeb.
① Qhov sib txawv ntawm cov enzymes: Feem ntau ntawm cov kev tshawb fawb hauv vitro siv cov nceb tyrosinase, uas nthuav tawm cov qauv thiab kev ua haujlwm sib txawv piv rau tib neeg tyrosinase. Ib qho zoo inhibitor ntawm nceb tyrosinase yuav txo tau cov nyhuv ntawm tib neeg tyrosinase, thiab vice versa. Yog li, Phloretin tuaj yeem pom qhov ua tsis tau zoo ntawm cov enzymes los ntawm ntau qhov chaw.
② Raw khoom purity thiab specifications: Raws li ib tug nroj tsuag extract,Phloretin covpurity, impurities, thiab cov txheej txheem rho tawm tag nrho cuam tshuam rau nws txoj haujlwm lom neeg. Kev ua haujlwm ntawm cov khoom siv raw los ntawm cov neeg muab khoom sib txawv tuaj yeem sib txawv heev.
③ Cov xwm txheej sim: Cov kev hloov pauv me me ntawm pH tus nqi ntawm qhov tsis, qhov kub thiab txias, substrate concentration, thiab lwm yam kev ntsuas yuav ua rau muaj kev hloov pauv hauv IC50 qhov tseem ceeb ntawm qhov kev txiav txim loj, thiab tseem ua rau cov lus xaus ntawm "zoo" lossis "tsis muaj txiaj ntsig".
2, Efficiency Duel: Kev sib piv ntau ntawm Phloretin thiab Classic Inhibitors
Txoj kev zoo tshaj los ntsuasPhloretin cov"lub zog" yog ncaj qha piv nws nrog kev lag luam- lees paub "cov qauv kub" xws li Kojic Acid thiab Arbutin.
- Phloretin vs Kojic Acid
Txoj kev tshawb no los ntawm Jianmin Chen et al. (2020) hais ua ntej muab kev sib piv ncaj qha. Hauv qhov kev sim no, quercetin tau siv los ua kev tswj hwm zoo nrog IC50 tus nqi ntawm 83.11 μ mol / L. Piv nrog Phloretin's 169.36 μ mol / L, qhov inhibitory kev ua ntawm quercetin yog kwv yees li ob npaug ntawm Phloretin. Qhov no qhia tau hais tias, los ntawm qhov kev xav ntawm inhibiting tyrosinase kev ua ib leeg, Phloretin yuav tsis zoo li quercetin.
Txawm li cas los xij, kev ua tau zoo tsis yog qhov kev xav xwb. Txoj kev tshawb no tseem hais ntxiv tias Phloretin muaj kev nyab xeeb dua. Vim nws muaj peev xwm cytotoxicity thiab stability teeb meem, quercetin raug rau qee yam kev txwv hauv nws daim ntawv thov. Yog li ntawd, Phloretin muab qhov sib npaug zoo dua ntawm kev nyab xeeb thiab kev ua tau zoo nrog nws cov khoom "mob siab thiab ua tau zoo".
- Phloretin vs. arbutin
Cov kev tshawb fawb tau pom tias hauv qee qhov system, IC50 tus nqi ntawm -arbutin yog 243.16 μM. Hauv lwm cov kev tshawb fawb, qhov inhibition ntawm arbutin ntawm tyrosinase (32%) tau qis dua li ntawm quercetin (62%). Zuag qhia tag nrho, arbutin feem ntau suav hais tias yog ib qho inhibitor me me. Txawm hais tias tsis muaj qhov sib piv ncaj qha, raws li IC50 tus nqi ntawm Phloretin 169.36 μ mol / L, nws qhov kev muaj peev xwm inhibitory tuaj yeem nyob rau tib theem li arbutin, lossis txawm tias zoo dua me ntsis. Txawm li cas los xij, qhov kev xav no xav tau cov ntaub ntawv kev sim ncaj qha ntxiv kom paub meej.
Kev ntsuam xyuas dav dav: Phloretin tus kheej li tyrosinase inhibitor tau tsim, thiab nws cov txiaj ntsig tau raug lees paub hauv kev tshawb fawb kev kawm, tab sis nws lub zog yuav tsis muaj zog dua li ntawm quercetin. Nws qhov tseeb kev lag luam kev sib tw tsis yog tsuas yog nyob rau hauv nws ib leeg inhibitory nyhuv ntawm tyrosinase, tab sis kuj nyob rau hauv nws multifunctionality - muaj zog antioxidant muaj peev xwm, "booster" nyhuv uas txhawb kev nkag mus ntawm lwm yam active tshuaj, thiab muaj kev ruaj ntseg zoo.
Hauv kev xaus, raws li cov nroj tsuag extract,Phloretinyog positioned raws li ib tug "powerhouse" es tsis yog ib tug "idol" nyob rau hauv daim ntawv ntawm whitening. Nws tus nqi yog tsis nyob rau hauv nws unparalleled inhibitory cov ntaub ntawv tab sis nyob rau hauv nws scientific proven ua tau zoo, txhim khu kev qha kev ruaj ntseg, thiab zoo heev formula synergy.
Yog xav paub ntxiv txogPhloretinthiabPhlorizin, txuas nrog Serrisha los ntawm APPCHEM. (Email:cwj@appchem.cn; +86-138-0919-0407)
Kev siv
[1] Phloretin ua ob qho tib si substrate thiab inhibitor ntawm tyrosinase: Inhibitory kev ua si thiab mechanism. Tsis tau teev tseg. [2020-02-05]
[2] Sustainable and Scalable Enzymatic Production, Structural Elucidation, Thiab Biological Evaluation of Novel Phlorizin Analogs. Laurène Minsat et al. [2024]
[3]Synthèse chimio-enzymatique, purification thiab caractérisation de molécules analogues aux métabolite secondaires du bois de co-produits de l'arboriculture pour des applications cosmétiques. Lauren MINSAT. [2022-11-10]
[4]EP 4 541 345 A1. European Patent Office[2025-04-23]
[5] Phloretin ua ob qho tib si substrate thiab inhibitor ntawm tyrosinase: Inhibitory kev ua si thiab mechanism. Jianmin Chen et al.[2018-10-19]
[6] PHLOROTANNINS thiab lawv cov cim kev lom zem. Chitikela P Pullaiah et al.
[7] Tyrosinase inhibitors cais los ntawm Ceratonia siliqua (L.) thiab Sideroxylon inerme (L.). Saeideh Momtaz. [2007-04]