Hauv kev lag luam cog qoob loo, txiv apples (Malus domestica) tau kawm dav rau lawv cov nplua nuj theem nrab metabolites xws lipolyphenols, flavonoids, thiab phenolic acids. Nyob rau hauv xyoo tas los no, ntau qhov kev sim cov ntaub ntawv tau pom tias cov txiv apples thiab lawv cov kev ua los ntawm - cov khoom (xws li cov tawv nqaij thiab cov txiv hmab txiv ntoo residue) muaj cov tshuaj tua kab mob tseem ceeb hauv vitro thiab hauv vivo, ua rau lawv yog "natural antibiotic".
1. Cov tshuaj tua kab mob tseem ceeb hauv cov txiv apples
- polyphenols
Anthocyanins, epicatechins, thiab quercetin muaj cov ntsiab lus siab tshaj plaws hauv cov kua txiv tev thiab nqaij. Kev tshawb fawb tau pom tias cov nopolyphenolstuaj yeem ua rau cov kab mob ntawm tes ua kom puas, inhibit enzyme kev ua haujlwm, thiab yog li ua tiav cov tshuaj tua kab mob.
- phenolic acid
Cov tshuaj xws li chlorogenic acid thiab caffeic acid tuaj yeem tsim cov hydrogen bonds nrog cov kab mob cov kab mob, cuam tshuam lawv txoj kev metabolic.
- organic acid
Malic acid, citric acid, thiab lwm yam, txo cov pH hauv zos thiab inhibit qhov kev loj hlob ntawm cov kab mob feem ntau.
- Volatile roj
Qhov me me ntawm aldehydes thiab ester volatile roj nyob rau hauv cov kua txiv hmab txiv ntoo nthuav tawm cov teebmeem inhibitory ntawm Staphylococcus aureus.
2. Kev sim tshuaj xyuas - hauv vitro tshuaj tua kab mob
| Hom kab mob | Kev kuaj xyuas txoj kev | Txoj kab uas hla ntawm antibacterial cheeb tsam (mm) |
| Staphylococcus aureus | Agar diffusion txoj kev | 19.25 ± 0.89 (txiv apples extract) vs 20.13 ± 0.83 (ciprofloxacin) |
| Escherichia coli | Agar diffusion txoj kev | 12.3 ± 0.7 (Kua pomace ethanol extract) |
| Pseudomonas aeruginosa | Micro dilution txoj kev | MIC =0.5 mg/mL (apple polyphenols) |
| Enterococcus faecalis | Micro dilution txoj kev | MIC =0.75 mg/mL (txiv apples extract) |
Cov ntaub ntawv no qhia tiastxiv apples extracttuaj yeem yuav luag sib tw cov tshuaj tua kab mob ciprofloxacin hauv inhibiting Staphylococcus aureus, thiab tseem muaj qhov cuam tshuam tseem ceeb ntawm cov kab mob gram- tsis zoo.
3. Nyob rau hauv vivo thwmsim thiab kev tshawb fawb preclinical
3.1 Qhov ncauj noj qab haus huv
Kev soj ntsuam randomized hauv Indonesia siv 12.5% kua tev extract qhov ncauj qhov ncauj ua rau txo qis ntawm kwv yees li 30% hauv cov quav hniav (PLI) yam tsis muaj kev phiv tshuaj.
3.2 Kev txuag zaub mov
Ntxivtxiv apples polyphenolsrau jam tuaj yeem cuam tshuam cov khoom noj uas muaj kab mob (Salmonella, Listeria) thiab ua kom lub neej txee ntev li 15 hnub thaum khaws cia ntawm 4 ° C rau 30 hnub.
3.3 Tsiaj Model
Hauv cov qauv nas ntawm kev mob plab hnyuv, qhov ncauj kev tswj hwm ntawm cov kua polyphenol extract tau txo qis cov kab mob plab hnyuv, thiab cov kab mob hauv cov ntshav (IL-6, TNF-) txo qis li ntawm 40%.
4. Daim ntawv thov scenarios
| Scenario | Cov khoom tshwj xeeb | Feature highlights |
| Kev txuag zaub mov | Kua polyphenols- natural preservatives (powder/liquid) | inhibit cov kab mob loj hlob, tiv thaiv oxidation, thiab txhim kho kev hnov lus zoo |
| Kev saib xyuas qhov ncauj | Kua tev extract- tshuaj ntxuav qhov ncauj, thiab tshuaj txhuam hniav additives | inhibiting hniav plaque thiab txo qhov ncauj o |
| Kev tu tawv nqaij | Kua polyphenol- daim npog ntsej muag, tes cream | tiv thaiv - kab mob, tiv thaiv - oxidation, txhawb kev kho tawv nqaij barrier |
| Tsi pub noj | Kua pomace hmoov (muaj polyphenols) | Inhibits pub pwm thiab txhim kho tsiaj tiv thaiv kab mob |
| Kev pab kho mob | Natural antibacterial excipient ntxiv rau cov tshuaj pleev hauv zos | Koom tes nrog cov tshuaj tua kab mob ib txwm siv los txo cov kev pheej hmoo ntawm cov tshuaj tiv thaiv |
Yog xav paub ntxiv txogKua extract thiab kua polyphenol, txuas nrog Serrisha los ntawm APPCHEM. (Email:cwj@appchem.cn; +86-138-0919-0407)
Kev siv
[1] Shanshan He, Gaopeng Yuan et al. "Major Latex Protein MdMLP423 Negatively Regulates Defense tiv thaiv kab mob fungal hauv Apple." International Journal of Molecular Sciences (2020). [2020-03-01]
[2] Bekämpning med "naturliga fungicider" mot lagringssjukdomar – Förstudie Tiv thaiv kev cia lwj los ntawm ntuj fungicides. Ibrahim Tahir.
[3]Txoj kev loj hlob ntawm cov nroj tsuag-raws li nanoemulsion thiab nws daim ntawv thov raws li ib tug natural preservative muaj antioxidant thiab antimicrobial zog kom xa active compounds nyob rau hauv kua txiv. Kailash Barman et al. [2022-12-13]